Part:BBa_K2967013
Amplifier system with hIL-10.
Amplifier system is created by our team NEU-CHINA 2019. To make sure that amplifier is able to strengthen the expression of gene downstream to promoter hrpL. A coomassie brilliant blue was demonstrated that more protein at 35kDA, which should be IL-10, was witnessed when introducing IL-10 gene downstream of promoter hrpL. This amplifier system tended to strengthen overall protein expression including the human IL-10.
Figure 1. Diagram of expressing vector. HrpR and HrpS amplifier system is used as a booster to boost the expression level of gene downstream promoter hrpL. Protein HrpR and HrpS can bind together to induce the PhrpL which strengthens the expression of downstream gene YebF-IL10.
Figure 2. Protein expression of human IL-10 gene which transformed in BL21 strain. CFU was then inoculated to LB medium followed by 12h incubation at 37℃. After induction of IPTG with the final concentration at 1mM, the culture was incubated at 37℃ for 2h. A coomassie brilliant blue was demonstrated to show the result. The red box shows where the target protein is.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2082
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1292
Illegal SapI.rc site found at 1925
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